Shahifatul Medina is one of the many verses on Human Rights, namely Surah Al Isra verse 70. The most fundamental statement of human rights in the history of Islamic civilization is the constitution of the world's first written agreement agreed upon Muhammad as the great leader of the Muslim community (Muhajirin and Ansar) with the Jewish population in Yathrib from various tribes and clans. This paper aims to describe the principle of Shahifatul Medina regarding human rights and the concept of religious moderation education. The results indicated that human rights in Medina charter referred to brotherhood, freedom, and equality and the concept of religious moderation education is found by correlating the three pillars of moderation: thought, action, and movement moderation.