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This study aims to explore and analyze gender discrimination practices in the Malo’ohamawa tradition in Gorontalo. The research location is in Gorontalo Province covering all districts and cities. Primary data used in this study are the results of interviews with sources and informants, in contrast secondary data are the results of research, journals, and books related to this research topic. This research is important because there has been no comprehensive research on the Malo’ohamawa tradition. This study uses a qualitative method with a philosophical anthropological approach. There are three most important findings in the study, first, the Molo'ohamawa tradition provides an experience of anxiety for every woman who will have sex on the first night of marriage. Secondly, in practice, the Malo’ohamawa tradition requires women to prove that their bodies are still virgins or have never been touched by men other than their husbands. This must be proven by the discharge of blood from the vagina. Thirdly, women receive social sanctions in the form of domestic violence, bad stereotypes from their husbands, and their discharge if on the first night, they are known to be no longer virgins or their vaginas do not bleed.