This study aims to determine the antituberculosis activity of Parang Romang root extract against Mycobacterium tuberculosis. Parang Romang root was extracted using 96% ethanol solvent by maceration method, the ethanol extract obtained was fractionated by the liquid-solid extraction method: obtain n-Hexan soluble extract and n-Hexan insoluble extract. Samples of 96% ethanol extract, soluble extract of n-Hexan and insoluble extract of n-Hexan were made in series of concentrations of about 250, 500, 750, and 1000 µg/ml. The inhibition test of Mycobacterium tuberculosis used the MODS method on the Mycobacterium tuberculosis strain H37RV. Color reagents then identify extracts that provide inhibition. The results of the minimum inhibition concentration of Mycobacterium tuberculosis showed that a 1000 µg/ml of n-Hexan insoluble extract was able to inhibit the growth of Mycobacterium tuberculosis strain H37RV. The group of compounds from the n-Hexan insoluble extract of Parang Romang root which is active as an antibacterial Mycobacterium tuberculosis is a class of alkaloid compounds.