Universitas Islam Negeri Alauddin Makassar Proceedings, Proceedings of the 1st Alauddin Health and Medical International Conference

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Anti inflammatory activities against LPS-induced J774.1 Cell and Total Flavanoid content of Vitex coffasus
Faradiba Abdul Raysid, Tifania Tifania, Abd. Malik

Last modified: 2022-09-24


Context: The secondary Metabolite compounds found in the vitex sp are terpenes, flavonoids, and lignans.  Flavonoids have shown anti inflammatory, anti-oxidative, anti-mutagenic, and anti-carcinogenic properties.  Vitex coffasus (VCS) research was limited to cancer activities and larvicides compared to other vitex species. Aim: This study aims to determine the anti-inflammatory activity against LPS-induced J774.1 cells. And the total flavonoid content of Vitex coffasus. Method: : Initially, the Vitex coffasus leaves were extracted by using methanol. Next, the extracts were divided into three concentrations 10, 30, and 100 µg/ml, and then evaluated Anti-inflammatory by using J774.1 cells function as a macrophage and after being triggered by LPS from Escherichia coli, these macrophages could produce Nitric oxide (NO).  Inhibition of NO was evaluated using Griess reagent.  The qualitative analysis of flavonoids used TLC and visualization by spraying with AlCl3.  The determination of total flavonoids is conducted based on the AlCl3 with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength  of 431 nm. Results: The extracts effectively reduced lipopolysaccharide (LPS)-induced NO levels in a 100 µg/ml concentration.  The total flavonoid content of VC leaf extract is 1.0318 mg QE / g extract.was 0,003817 mgQE/g extract. Conclusion: From these results, we can conclude that the VCS leaves possess the anti inflammatory activity and total flavonoid content was 0,3817%