This study aims to develop e-book learning media using the Kvisoft Flipbook Maker application and evaluate its validity, practicality, and effectiveness. This research falls under Research and Development (R&D) using the ADDIE development model, which has five stages (analysis, design, development, implementation, and evaluation). The product went through three stages of testing: validity, practicability, and effectiveness. The subjects in this research were 21 Class B students from the Class of 2021 Department of Biology Education, Alauddin State Islamic University, Makassar. The research instruments used are validation sheets to determine the validity of the product to be developed, student response questionnaires and lecturer response questionnaires to determine the practicality of the product to be developed, and learning outcomes tests to determine the effectiveness of the product to be developed. The research results showed that the developed media has a validity level of 3.8, which refers to very valid; a practical level of assessing lecturer and student responses is 3.5, which means very practical; and the learning outcomes test achieved 100% with an average score of 84.1, indicating that e-book learning media meets the criteria of being valid, practical, and effective. This research suggests that e-books can enhance student learning activities.