Religious maturity is the maturity of faith that is formed through a person's experience in living a religious life throughout his life which is based on noble values ​​and influences his attitude and behavior. Understanding the values ​​of religious teachings is very much needed in facing the challenges of the era of society 5.0. Changing times and increasingly advanced technology impact the world of education, especially regarding teachers' ability to teach. One aspect is a teaching culture which will lead a teacher to have quality work attitudes and behavior and always be based on meaningful values ​​so that they become motivated, and provide inspiration to always work better and more satisfactorily. This research aims to determine the influence of religious maturity on the teaching culture of PAI teachers in Banjarnega Regency. The research method used is quantitative with data collection techniques using questionnaires and data analysis techniques using simple regression tests. The results of this research discuss the religious maturity of PAI teachers in Banjarnegara Regency in the high category with a percentage value of 70%. The teaching culture of teachers in the era of society 5.0 is in the medium category with a percentage score of 50%. The calculated F value (2.498) is greater than the F table (F(0.05.3/30) = 2.032) Sig value. in the table, the value is 0.402 < 0.05 so it can be concluded that there is a significant influence of religious maturity on the teaching culture of PAI teachers in Banjarnegara Regency in the era of society 5.0. This explains that the higher the religious maturity value, the teacher's teaching cultural value will be higher or increased, and vice versa. The teaching culture of teachers in the era of Society 5.0 can be used as a guide for a teacher to realize better performance through good social attitudes and spiritual attitudes through his religious maturity.